The Gloucester-Hartpury second row debuted in a 2016 Summer Tour victory over France and was awarded World Player of the Year five years later.
She retains her place in the number four jersey as head coach John Mitchell makes seven changes from the opening round win against Italy.
Tatyana Heard and Megan Jones form a new centre partnership while Natasha Hunt and Holly Aitchison pair up in the half-back positions.
Maud Muir and Rosie Galligan come into the front five with Alex Matthews promoted from the bench to Number 8.
The back three of Jess Breach, Abby Dow and Ellie Kildunne remains consistent. Hannah Botterman, Lark Atkin-Davies, Sadia Kabeya and centurion Marlie Packer also continue their starting roles.
The Red Roses will break another attendance record when they take to the field on Saturday by taking to the field in front of their largest ever home crowd in a fixture not played at Twickenham Stadium.
Red Roses side to play Wales
15 Ellie Kildunne (Harlequins, 39 caps); 14 Abby Dow (Trailfinders Women, 41 caps); 13 Megan Jones (Leicester Tigers, 17 caps), 12 Tatyana Heard (Gloucester-Hartpury, 18 caps); 11 Jess Breach (Saracens, 34 caps); 10 Holly Aitchison (Bristol Bears, 26 caps), 9 Natasha Hunt (Gloucester-Hartpury, 68 caps); 1 Hannah Botterman (Bristol Bears, 43 caps), 2 Lark Atkin-Davies (Bristol Bears, 55 caps), 3 Maud Muir (Gloucester-Hartpury, 26 caps); 4 Zoe Aldcroft (Gloucester-Hartpury, 49 caps), 5 Rosie Galligan (Saracens, 14 caps); 6 Sadia Kabeya (Loughborough Lightning, 14 caps), 7 Marlie Packer (Saracens, 100 caps) (captain), 8 Alex Matthews (Gloucester-Hartpury, 63 caps)
Replacements
16 Connie Powell (Harlequins, 15 caps), 17 Mackenzie Carson (Gloucester-Hartpury, 11 caps), 18 Kelsey Clifford (Saracens, 4 caps), 19 Abbie Ward (Bristol Bears, 62 caps), 20 Maddie Feaunati (Exeter Chiefs, 1 cap), 21 Lucy Packer (Harlequins, 17 caps), 22 Zoe Harrison (Saracens, 47 caps), 23 Sydney Gregson (Saracens, 3 caps)
